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B-Fahf-2 Pretreatment Reduces OIT Adverse Reactions and Improves Outcomes In a Murine Model Of Multiple Nut Allergy
Sunday, March 2, 2014: 3:45 PM
Ballroom 6B (Convention Center)
Xiu-Min Li, MD, , , , ,
Rationale: Adverse reactions during OIT for food allergy are a central concern and barrier to wide clinical use. The TCM herbal formula B-FAHF-2 completely protects mice from anaphylaxis. We questioned whether pretreatment with B-FAHF-2 could improve safety profile and outcome of OIT for multiple nut allergy (MNA).

Methods: C3H/HeJ mice were concurrently sensitized to peanut (PN), walnut (WN) and cashew (CSH). Oral challenge with individual nuts induced anaphylactic reactions. Some multiple nut allergic mice then received twice daily B-FAHF-2, control mice received sham treatment. OIT using a PN/WN/CSH mixture was then performed. After completing OIT all mice were orally challenged twice with a PN/WN/CSH mixture, and then with individual nuts. Symptom scores and core body temperatures were recorded on day 1 of OIT and after each challenge. Histamine, nut-specific IgEs, and cultured splenocyte cytokines were measured by ELISA.

Results: BFAHF-2 treated mice exhibited fewer OIT adverse reactions, lower symptom scores and serum histamine levels than sham-treated mice (P<0.05-0.01 for all). At post-OIT challenges treated mice exhibited lower levels of nut-specific IgEs, fewer and milder reactions and lower histamine levels than control mice (P<0.05-0.01). IFN-gamma/IL-13 and IL-10/IL-13 ratios in cell cultures from B-FAHF-2 treated MNA mice but not control mice were elevated (P<0.05).

Conclusions: BFAHF-2 pretreatment reduced adverse reactions during OIT, and produced greater post-OIT protection and a beneficial immunregulation. Addition of BFAHF-2 to an OIT regimen for human food allergy may improve its safety and efficacy.