Expression of ADAM17 and ADAM10 in Nasal Polyps
Saturday, March 5, 2016
South Exhibit Hall H (Convention Center)
Woo Yong Bae, MD, Seong Kook Park, MD, Dae Young Hur, MD, Tae Kyung Koh, MD, Ji Won Seo, MD
Rationale: The “a disintegrin and metalloproteases” (ADAMs) are a multi-functional gene family that contributes to the homeostasis of the extracellular matrix, transduction of specific intracellular signals, organogenesis, inflammation, tissue remodeling, adhesion, and cell migration. ADAM17 is the best characterized of the sheddases, and its putative substrates are widespread, including various inflammatory modulators. ADAM10 is the most similar to ADAM17 in terms of protein sequence and the structural properties of their catalytic domains. Objectives: To assess the roles of ADAM17 and ADAM10 in nasal polyps (NPs) by measuring their expression.

Methods: The expression of ADAM10 and 17 was investigated in NPs at endonasal sinus surgery (n=15) and compared with that in inferior turbinate mucosa samples obtained from non-allergic hypertrophic rhinitis patients (n=15). Tissue samples were analyzed by real time polymerase chain reaction (PCR), Western blotting, and immunohistochemical staining.

Results: The ADAM17 mRNA and protein levels were significantly higher in the inferior turbinate than in NP (P < 0.05). The ADAM10 mRNA and protein levels did not differ significantly between NP and the inferior turbinate (P > 0.05). ADAM10 and 17 were expressed mainly in inflammatory cells, submucosal glandular cells, and lining epithelial cells.

Conclusions: We suggest that decreased ADAM17 expression contributes to the development of NPs and abnormal structural tissue remodeling in NPs.