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Serum Cytokine Profiles in Acute Exacerbation of Asthma Subtypes Measured By Cba Method
Sunday, March 6, 2016
South Exhibit Hall H (Convention Center)
Zhihui Min, Zhihong Chen, Xiaoqiong Su, Chunlin Du, Xiangdong Wang
Rationale: Cytokines play a critical role in the pathogenesis of asthma. In this study, cytokines in serum were detected for chronic asthma and acute exacerbation by cytometric beads array method (CBA).  Characteristics of cytokine subgroup and their relation to clinical data were analyzed.

Methods: Fourty chronic asthma, 22 acute exacerbation of asthma and 18 healthy subjects were included. A little amount of blood was drawn from each subject (2ml) and used for measure cytokines profiles, regular blood test, C reaction protein and procalcitonin.

Results: Cytokine panels were custom-made ,which included IL-5,IL-6,IL-10,IL-13,IL-17,TNF-α,IFN-γ. No differences were found in cytokine levels in chronic asthma when compared with healthy subjects. However in acute exacerbation phase, there were 5 subtypes which were high IL-6”(22.8%),“high IL-6/TNF-α”(13.6%),“high IL-5” (31.8%),“high IL-17”(13.6%),“Normal cytokine”(18.2%). The levels of IL-6, TNF-αsoared significantly, by 6.28~12 foldsA17.3 folds respectively. IL-5 and IL-17 increase slightly, which were 3 folds and 2.01 folds respectively when compared with healthy. “high IL-6”,”high IL-6/TNF-α” were positively correlated with the rise of WBC and CRP.

Conclusions: CBA was a cost-effective and time-consuming way to detect muti-cytokines in small volume sample. Although no differences were found in cytokine levels in chronic asthma, the specific cytokine panel may be helpful to identify different subgroup of acute exacerbation in asthma which helps indicate the cause of AE and guide appropriate antibiotic therapy.