Cockroach Protease Induces Allergic Airway Inflammation Via IL-33 and TSLP Secretion By Epithelial Cells
Saturday, March 5, 2016
South Exhibit Hall H (Convention Center)
Naveen Arora, Ph.D, Sagar Laxman Kale, Komal Agrawal, Shailendra N. Gaur, MD FAAAAI
Rationale: Periplaneta americana protease activates epithelial cells and initiates allergic airway inflammation at mucosal surfaces. The present study aimed to investigate the role of protease activity in airway inflammation. 

Methods: To check the effect of its activity, BEAS-2B cells were exposed to proteolytically active or inactive Per a 10 and secretion of proinflammatory cytokines was assessed in the supernatant. Per a 10 (active and inactive) was administered i.n. to mice for three times a week for two weeks and were sacrificed to analyse Immunoglobulin levels in sera, cytokines and uric acid in BALF. The levels of cytokines and uric acid were assessed in the cockroach hypersensitive patient’s sera.

Results: Per a 10 induces secretion of TSLP and IL-33 from BEAS-2B cells in an activity dependent manner. They also demonstrated time dependent increase in ATP on exposure to Per a 10. Intranasal administration of active Per a 10 resulted in elevated cellular infiltration and Th2 cytokines in BALF and serum IgE as compared to inactive and PBS immunised mice. Levels of TSLP and IL-33 in the BALF of active Per a 10 administered mice were elevated as compared to inactive Per a 10 and PBS administered mice. Mice administered with active Per a 10 showed higher uric acid levels. Analysis of cockroach hypersensitive patient’s sera revealed higher levels of IL-33 and uric acid as compared to healthy subjects. 

Conclusions: Proteolytic activity of Per a 10 causes airway inflammation by elevation of TSLP, IL33 and uric acid levels.