Microparticles in Nasal Lavage; Potential Biomarkers for Chronic Rhinosinusitis and Aspirin Exacerbated Respiratory Disease
Sunday, March 6, 2016: 4:45 PM
Concourse Foyer (Convention Center)
Toru Takahashi, MD PhD, James E Norton, MS, Lydia Suh, BSc, Roderick G. Carter, BSc, Robert C. Kern, MD, Bruce K. Tan, MD, Stephanie S. Smith, MD, Kevin C. Welch, MD, David B. Conley, MD, Anju T. Peters, MD FAAAAI, Leslie C. Grammer, MD FAAAAI, Kathleen E. Harris, BSc, Whitney W. Stevens, MD PhD, Kathryn E. Hulse, PhD, Bruce S. Bochner, MD FAAAAI, Atsushi Kato, PhD, Robert P. Schleimer, PhD FAAAAI
Rationale: Microparticles (MPs) are shed membrane vesicles that are released when cells are activated or apoptotic. The released MP levels reflect degree of either cell injury, cell activation or both. We tested whether MPs are released into nasal cavity in response to the pathophysiology of chronic rhinosinusitis without nasal polyps (CRSsNP), with NP (CRSwNP), and Aspirin Exacerbated Respiratory Disease (AERD).

Methods: 23 CRSsNP, 23 CRSwNP, 18 AERD patients and 15 control subjects were enrolled. Nasal lavage fluids were collected, processed, and analyzed for MP using FACS. MPs were defined as follow; E-cadherin+MPs (Epithelial MPs), E-selectin+MPs (Activated endothelial MPs), EMR-1+MPs (Eosinophil MPs) and FceRI+/c-kit+MPs (Mast cell MPs). Eosinophil cationic protein (ECP) levels were measured by ELISA.

Results: When compared with control, E-cadherin+MPs were increased in CRSsNP (2 fold vs. control, p<.05), and AERD (1.5 fold, p<.05) but not in CRSwNP. E-selectin+MPs were increased in CRSsNP (1.5 fold, p<.05), CRSwNP (1.5 fold, p<.05) and AERD (3 fold, p<.01). EMR-1+MPs were increased in CRSsNP (2 fold, p<0.001), CRSwNP (1.5 fold, p<.05) and AERD (2.5 fold, p<0.001). There was a trend toward elevated levels of FceRI+/c-kit+MPs, but it was not significant. There was a significant correlation between ECP and EMR-1+MPs only in CRSwNP (rs=0.513, p<.05).

Conclusions: These results demonstrate an elevation of epithelial, endothelial and eosinophil MPs, along with a trend toward increased levels of mast cell MP, in CRS patients, and support previous findings of activation of these cells in CRS. MP analysis may be valuable in the study of CRS, both in the laboratory and the clinic.