Methods: Source materials and allergenic extracts were evaluated for total protein (Bradford) and protein/antigenic profiling (WES Nanotechnology Capillary Electrophoresis from ProteinSimple, Santa Clara, CA, or conventional SDS-PAGE / Western Blotting). WES kits contain sample preparation reagents, pre-filled plates with proprietary electrophoretic reagents, disposable capillary cartridges, and all post-sample preparation steps (loading, washing, immunoprobing, data analysis, incubation time) are fully automated.
Results: Both protein and antigenic patterns were qualitatively similar between WES and the traditional techniques. WES provided several additional benefits, including small reagent volume (5-10 µL), quantitation, high throughput (3-4 hour run times), complete automation of reagents and assay conditions, analytical integrations, matrix-based molecular weight determination, and real-time migration visualizations.
Conclusions: WES assays were simple to perform and provided both similar qualitative data compared to traditional methods and useful quantitative data with full automation. This new nanotechnology allows for a higher throughput of raw material and allergenic extract samples using 5-10 µL of each reagent.