Relationship of Der f1 and Der p1 Levels in House Dust in the Midwestern US
Saturday, March 5, 2016
South Exhibit Hall H (Convention Center)
Charles S. Barnes, PhD FAAAAI, Freddy Pacheco, MS, Jay M. Portnoy, MD FAAAAI
Rationale: Two species of house dusty mite (Dermatophagoides farinae and D. pteronyssinus) are common in the Midwestern US. Knowledge of measured levels of house dust mite allergenic proteins in vacuumed house dust can be a helpful parameter when evaluating home allergen avoidance and remediation strategies. To evaluate the relationship of Der f1 levels to Der p1 levels we conducted the following studies

Methods: House dust was collected from homes in the Kansas City Metropolitan area that participated in 4 studies of allergens in housing and schools during the past 7 years. Between 1 and 3 dust samples were collected per house. House dust was analyzed for levels of Der f1 and Der p1 allergen using specific monoclonal antibodies and commercially available kits (InBio). Values were reported as nanograms per gram of dust.

Results: We analyzed 1353 house dust samples over the period of 7 years. The maximum Der f1 level was 86,778 and the maximum Der p1 level was 13,743. The Mean Der f1 level was 378 ±2438 and the mean Der p1 level was 248±602. Der f1 was above the level of detection in 95% of samples and Der p1 was above the LOD for 97% of samples. The correlation between Der f1 levels and Der p1 levels was 0.08.

Conclusions: Most homes contained measurable levels of both Der f1 and Der p1 allergen proteins. The moderate but positive correlation between the two mite allergens indicates that conditions conducive to the growth of both types of mites occur in many homes.