Methods: The devices were run at the same time at 12 locations in a home occupied by a family and a dog. The dog had access to all rooms except the basement. Twelve common household allergens were tested with MARIA™ multiplex immunoassays kits from Indoor Biotechnologies. Biomass measurements on the same samples were performed by spectrophotometry. Allergen and biomass capture increased linearly for 5 days and plateaued between 5 and 7 days. Five days was therefore selected as the standard sampling time.
Results: Allergen profiles showed similar levels of Can f 1 (dog) at all testing locations except for a trace found in the basement from where the dog was excluded. Mold Asp f 1 was found in the basement but in no other location. Four locations showed timothy grass at borderline detectable levels. No other allergens were detected. Biomass was within a cv of +14%, showing relative consistency of air quality throughout the house.
Conclusions: Outdoor allergens may appear sporadically. Results are consistent with a dynamic equilibrium throughout the house depending on air flow and pet movements. Home aeroallergen sampling from any of several locations may accurately reflect a person’s allergen exposure.