Methods: We recruited 42 pediatric non-IgE-GI milk allergy patients together with 10 IgE-dependent milk allergy patients, and obtained written informed consent. Plasma samples were obtained before the resolution of the GI symptoms. Plasma cytokine/chemokine levels were measured by multiplex assay.
Results: Similar levels of plasma Th2 and Th17 cytokines (IL-5, IL-13 and IL-17) were observed in non-IgE-GI-FA and IgE-FA. The MCP-2/CCL8 level was significantly higher, whereas the MIP-1β/CCL4 level was significantly lower, in non-IgE-GI-FA compared with IgE-FA.
Conclusions: Our results suggest that the mechanisms of lymphocyte recruitment in non-IgE-mediated allergy may be distinct from those in IgE-mediated allergy, although both allergy types have a similar Th2-predominant pathogenesis. Further studies are required to elucidate the molecular and cellular mechanisms underlying release of these chemokines from the affected tissues.