Plasma Cytokine/Chemokine Profiles in Non-IgE-Mediated Gastrointestinal Food Allergy
Monday, March 7, 2016
South Exhibit Hall H (Convention Center)
Kanami Orihara, PhD, Ichiro Nomura, MD, PhD, Tetsuo Shoda, MD, PhD, Hideaki Morita, MD, PhD, Hiroko Suzuki, MD, PhD, Akio Matsuda, PhD, Hirohisa Saito, MD PhD FAAAAI, Kenji Matsumoto, MD, PhD
Rationale: Reports of non-IgE-mediated gastrointestinal food allergy (non-IgE-GI-FA) cases have increased in recent decades.  We previously reported that the antigen-specific cytokine secretion profile of PBMCs from non-IgE-GI-FA patients is Th2-predominant. Also, massive eosinophilia was observed in approximately 70% of biopsy specimens from those non-IgE-GI-FA patients.  However, it remains unclear how lymphocytes are recruited to the GI tract in those patients and to the skin in IgE-mediated food allergy (IgE-FA) patients.  We examined plasma cytokine/chemokine levels in order to elucidate the Th2-predominant pathogenesis shared by non-IgE-GI-FA and IgE-FA and to identify differences in lymphocyte recruiting factors between non-IgE-mediated and IgE-mediated allergies.

Methods: We recruited 42 pediatric non-IgE-GI milk allergy patients together with 10 IgE-dependent milk allergy patients, and obtained written informed consent.  Plasma samples were obtained before the resolution of the GI symptoms.  Plasma cytokine/chemokine levels were measured by multiplex assay.

Results: Similar levels of plasma Th2 and Th17 cytokines (IL-5, IL-13 and IL-17) were observed in non-IgE-GI-FA and IgE-FA.  The MCP-2/CCL8 level was significantly higher, whereas the MIP-1β/CCL4 level was significantly lower, in non-IgE-GI-FA compared with IgE-FA.

Conclusions: Our results suggest that the mechanisms of lymphocyte recruitment in non-IgE-mediated allergy may be distinct from those in IgE-mediated allergy, although both allergy types have a similar Th2-predominant pathogenesis.  Further studies are required to elucidate the molecular and cellular mechanisms underlying release of these chemokines from the affected tissues.