Down-Regulated the Expression of Wdpcp Could Interrupt the Ciliogenesis in CRS
Saturday, March 5, 2016
South Exhibit Hall H (Convention Center)
Yinyan Lai, Yun Ma, Jianbo Shi
Rationale: Cilia loss is one of the significant morphologic changes in sinonasal mucosa from patients with chronic rhinosinusitis (CRS). However, the mechanism for poor ciliation is still unclear.We sought to find out the role of WDPCP, a key factor for ciliogenesis in recent researches, in human sinonasal epithelia cells cilia loss from patients with CRS. Methods: We investigated the expression of WDPCP in human sinonasal epithelium from patients with CRS, as well as comparing them with control subjects. Meanwhile, we used primary human sinonasal epithelial cells in air-liquid interface (ALI) in vitro model to observe WDPCP's expression during ciliogenesis. Results: Decreased expression of WDPCP with cilia loss was detected in sinonasal mucosa from patients with chronic rhinosinusitis. In vitro experiments, we found that WDPCP level upregulated at first, and then declined. Knocking down WDPCP expression at the beginning of differentiation would reduce the quantity and length of cilia. Also, the stimulation of Th1 inflammatory cytokines could downregulate the expression of WDPCP. Conclusions: WDPCP plays an important role in ciliogenesis of human airway, and it may also contribute the pathogenesis of CRS.