The Measure of Specific IgE to Whole-Allergen Extracts May Not be Useful for Primary Sensitization Diagnosis in Children with Atopic Dermatitis and Asthma
Sunday, March 6, 2016
South Exhibit Hall H (Convention Center)
Lukasz Blazowski, MD PhD, Ryszard Kurzawa

Children with atopic dermatitis and asthma are mainly polysensitized thus use of specific IgE to allergen extracts in sensitization diagnosis may lead to misdiagnosis. In this cross-sectional, prospective study the degree of cross-reactivity using component resolved diagnostics (CRD) was analyzed.


Serum specific IgE to 112 allergen components were measured by using multiplex microarray (ISAC) in 297 sensitized children (aged 1-18 years) with atopic dermatitis (mAD-moderate, sAD-severe, objective SCORAD index 15-40 and >40 respectively), asthma (A1-episodic, A2-mild persistent or A3-moderate) or both. Sensitization to 7 main cross-reactive allergen families (C-RAF) and specific IgE to cross-reactive carbohydrate determinants (CCD) was identified and evaluated depending on clinical diagnosis and age.


IgE reactivity to C-RAF and CCD was found in 81,1%  of children (52,9%, 17,5%, 14,8%, 4,7%, 52,2%,  21,6%,  14,5%  and 9,4%  in pathogenesis-related class 10 proteins, non-specific transfer lipid proteins, profilins,  polcalcins,  lipokalins, serum albumins, tropomyosins  and CCD respectively. Depending on clinical diagnosis IgE to C-RAF was found in 75,3% children with asthma (57,9%, 78,9% and 85,0% in A1, A2 and A3 respectively), in 76,0% with atopic dermatitis (61,1% in mAD and 87,3% in sAD) and in 92,6% children with both atopic dermatitis and asthma. IgE reactivity to C-RAF was different depending on age: in 60,8%, 84,1% and 92,8%  of children at age 1-2, 3-6 and 7-18 years respectively.

Conclusions: In children with atopic dermatitis and/or asthma CRD is essential to  due to widespread sensitization to C-RAF and high cross-reactivity to components within each family.