Evaluation of Antibody, Cytokine and Mirnas in Patients with Ige-Mediated and Non-IgE Mediated Rhinitis in Brazilian Subjects Triggered By House Dust Allergen Exposure

Rationale: The aims were to investigate antibody responses to Dermatophagoides pteronyssinus (Dp), cytokine levels, relative expression of mRNA cytokines and microRNA (miRNA) in patients with rhinitis symptoms triggered by HDM exposure and with or without systemic IgE (S-IgE-) and control (CT) subjects.

Methods: Groups divided in S-IgE+ (n = 32), S-IgE- (n = 19), and CT (n = 31) by clinical symptoms, skin prick test (SPT) and specific IgE to Dp were analyzed. Serum IgG1, IgG3, IgG4, and IgA specific to crude Dp allergen were measured by ELISA. PBMCs from subjects (S-IgE+,15;S-IgE-,11;CT,10) were isolated and stimulated with Dp and mitogen or medium for controls. Supernatants for evaluating the cytokine levels, harvested cells for mRNA cytokines and miRNA by RT-qPCR.

Results: Serum IgE and IgG4 anti-Dp showed significantly higher in S-IgE+ than S-IgE- or CT subjects. IgG3 to Dp showed significant higher levels in S-IgE+ than CT group. Dp-specific IgA and IgG1 were not different among the groups. IL-5 was significantly higher in supernatants after Dp-stimulation from S-IgE+ than S-IgE- or CT groups but not for IL-4, IL-10, IL-13, IL-17 and IFN-g levels. Higher IL-5 levels in S-IgE+ compared to S-IgE- and CT groups and higher IFN-g levels were found in S-IgE+ compared to CT; and higher TGF-b expression in S-IgE- group. miRNAs expression was similar among the groups.

Conclusions: Data suggest that the mechanism involved in S-IgE- is different from S-IgE+ group, and our findings showed that antibody, cytokine and miRNAs profiles analyzed in this study were not able to discriminate these groups.