Inhibition of Inflammation and Mucus Production By Bordetella Pertussis Whole-Cell Vaccine in a Murine Model of Allergic Rhinitis
Saturday, March 5, 2016
South Exhibit Hall H (Convention Center)
Marcelo Vivolo Aun, MD, Fernanda Arantes-Costa, Francine Maria Almeida, Thayse Regina Brüggermann, Beatriz Mangueira Saraiva-Romanholo, Isabella S Genaro, Milton Arruda Martins, MD, PhD, Jorge Kalil, MD, PhD, Pedro Giavina-Bianchi, MD PhD
Rationale: Bordetella pertussis whole-cell vaccine (Pw) has shown to inhibit inflammation in animal models of asthma induced by ovalbumin and aeroallergens. We evaluated the effects of diphtheria-tetanus-pertussis (DTPw) vaccine in a murine model of allergic rhinits induced by Dermatophagoides pteronyssinus(Derp).

Methods: The protocol lasted 30 days. BALB/c mice were divided into 6 groups, which were sensitized subcutaneously (s.c.) with saline solution or Derp 50mcg, in three injections. Three groups were submitted to saline, with or without diphtheria-tetanus (DT) and DTPw vaccines. The other three groups received Derp with or without DT and DTPw vaccines. Subsequently mice underwent intranasal challenge with saline or Derp for 7 days and were sacrificed 24h after the last challenge. We measured serum specific IgE, the density of leukocytes infiltration in nasal mucosa and we measured the acidic nasal mucus content.

Results: Animals sensitized with Derp produced specific IgE, which was decreased by both vaccines. Derp+DTPw group developed lower levels of IgE anti-Derp (p<0.05), in comparison to Derp and Derp+DT groups. Furthermore, DT and DTPw vaccines decreased density of polymorphonuclear leukocytes infiltration in comparison to Derp group (p<0.05). We also found that both vaccinated groups had lower levels of acidic nasal mucus (p<0.05). Moreover, Derp+DTPw group had lower quantities of acidic nasal mucus than Derp+DT group (p<0.05).

Conclusions: In this murine model of allergic rhinitis, DTPw vaccine decreased serum specific IgE, inflammatory infiltrate and acidic mucus in upper airways. Pw vaccine should be evaluated as an option to induce immune regulation in respiratory allergy.