Relationship Between Serious Wheat Allergy Caused By Cutaneous Sensitization and Mutations in the Filaggrin Gene
Sunday, March 6, 2016
South Exhibit Hall H (Convention Center)
Akiko Yagami, MD, PhD, Emiko Noguchi, MD, PhD, Mayumi Tamari, MD, PhD, Tomomitsu Hirota, DDS PhD, Zenichiro Kato, MD, PhD, Hirohisa Saito, MD PhD FAAAAI, Kayoko Matsunaga, MD, PhD
Rationale: Many cases of wheat allergy developing after use of a soap containing hydrolyzed wheat protein (Glupearl 19S (GP19S) used in “Cha no Shizuku”, Yuuka Co., Ltd.) have occurred in recent years in Japan. This allergy is thought to occur due to percutaneous sensitization, but its pathogenic mechanism has not yet been elucidated. On the other hand, loss-of-function mutations in the filaggrin gene have been reported to be strongly associated with the onset of atopic dermatitis. The objective of the present study was to elucidate the relationship between the filaggrin gene, which affects skin barrier function, and this wheat allergy.

Methods: A genetic association study was conducted on the loss-of-function mutations in the filaggrin gene in Japanese individuals, using DNA for genomic analysis of patients who developed this serious wheat allergy due to GP19S (n=480).

Results: The loss-of-function mutations in the filaggrin gene, 3221delA, Ser2554X, Ser2889X, Ser3296X, and Lys4022X, were genotyped for a total of 480 patients, and allele frequencies of the patients were compared with those of Japanese general population. Loss of function mutation was observed in 50 patients (allele frequency = 0.052) and no significant differences were observed between cases and controls (P > 0.1).

Conclusions:  No associations were found between the serious wheat allergy caused by GP19S and loss-of-function mutations in the filaggrin gene. Further studies are needed to elucidate the genetic component of the disease.