Methods: A genetic association study was conducted on the loss-of-function mutations in the filaggrin gene in Japanese individuals, using DNA for genomic analysis of patients who developed this serious wheat allergy due to GP19S (n=480).
Results: The loss-of-function mutations in the filaggrin gene, 3221delA, Ser2554X, Ser2889X, Ser3296X, and Lys4022X, were genotyped for a total of 480 patients, and allele frequencies of the patients were compared with those of Japanese general population. Loss of function mutation was observed in 50 patients (allele frequency = 0.052) and no significant differences were observed between cases and controls (P > 0.1).
Conclusions: No associations were found between the serious wheat allergy caused by GP19S and loss-of-function mutations in the filaggrin gene. Further studies are needed to elucidate the genetic component of the disease.