Wnt Signaling in Nasal Polyp
Saturday, March 5, 2016
South Exhibit Hall H (Convention Center)
Ji-Hun Mo, Young-Jun Chung, Yun-Hee Rhee
Rationale: Wnt signaling is  important in  carcinogenesis, however it was rarely studied in the field of immunology. Recently it was identified that wnt signaling impairs regulatory T cell function. This study was aimed to evaluate wnt signaling and its association of regulatory T cell in nasal polyp.

Methods: Twently two patients with chronic rhinosinusitis with nasal polyposis (CRSwNP) were enrolled and divided into eosinophilic nasal polyps (EPs) and non-eosinophilic nasal polyps (NEPs) according to the proportion of eosinophils. Ten subjects with chronic hypertrophic rhinitis who underwent turbinoplasty were enrolled as control subjects. Expressions of CD25, FOXP3, IL-17, STAT3, Wnt3a and beta -catenin were measured quantitatively after immunofluorecent staining with confocal microscope. And  PCR array targeting wnt-related genes was also performed.

Results: Expressions CD25 and FOXP3 were significantly lower in EPs than NEPs or control. IL-17 expression levels were also increased in Eps than NEPs or control, showing inverse correlation between FOXP3 and IL-17. STAT3 did not show any significant difference between groups. Wnt3a did not show significant difference between groups, however, beta-catenin which is a target molecule of wnt signaling was increased in bothe EP and NEPs compared with control mucosa. RT2 PCR array also showed that Wnt2B, Wnt3A, Wnt5B, Wnt7B, Wnt8A, Fzd, and TCF were increased in EP and that Wnt2B, Wnt5B, Wnt7B, Wnt8A and TCF were increased in NEP, suggesting the role of Wnt signaling in nasal polyps. 

Conclusions: Wnt signaling, which might explain decreased FOXP3 expression, was increased in nasal polyps and may play a key role in the pathogenesis of nasal polyps.