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The C159T Polymorphism of the CD-14 Gene in Adult Patients with Corticosteroid-Sensitive and Refractory Bronchial Asthma in Crimea, Ukraine
Sunday, March 6, 2016
South Exhibit Hall H (Convention Center)
Yuri Bisyuk, Andrey I. Kurchenko, A.I. Dubovyi, Ganna V. Bisyuk, Lawrence M. DuBuske, MD, FAAAAI
Rationale: C-to-T transition has been identified at position 159 in the promoter region of the CD14 gene. This functional single-nucleotide polymorphism, C159T has been associated with asthma and allergic rhinitis in various ethnic populations. This association has not yet been confirmed in patients with refractory or corticosteroid-sensitive asthma in Ukrainian population. 

Methods: C159T polymorphism of CD-14 receptor gene was assessed in 40 patients with refractory and 291 with corticosteroid-sensitive asthma.  The control group included 285 non-atopic volunteers. Single nucleotide polymorphism of C159T was detected by PCR. Patients and volunteers were recruited at the Crimean State Medical University, Simferopol, Crimea, Ukraine.

Results: In the control group, the frequency distribution of genotypes (CC – 97 (34%), CT – 146 (51%), TT – 42 (15%)) was not significantly different from asthma with refractory (CC – 21 (52%), CT – 14 (35%), TT – 5 (13%), χ2 = 5.292, p = 0.070) versus corticosteroid-sensitive (CC – 84 (29%), CT – 155 (53%), TT – 52 (18%), χ2 = 2.204, p = 0.332) phenotypes. The risk analysis for the T allele ([СС]<->[CT+TT]) showed that the frequency of the genotype CT + TT in patients with refractory asthma (49%) was significantly lower (OR = 0.467, CI = [0.240-0.910], χ2 = 5.17, p = 0.023) compared to the control group (66 %).

Conclusions: The C159T polymorphism of CD14 is associated with refractory asthma in the Crimean population of Ukraine.