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Cytokine Profiles in Monosensitized and Polysensitized Allergic Rhinitis Patients Treated with Sublingual Immunotherapy
Monday, March 7, 2016
South Exhibit Hall H (Convention Center)
L. Maslova, Leonid P. Titov, MD, PhD, Lawrence M. DuBuske, MD, FAAAAI
Rationale: Cytokines induce allergic inflammation leading to respiratory allergy. This study assesses intracellular cytokine profiles in patients receiving Sublingual immunotherapy (SLIT).

Methods: 60 adult patients with allergic rhinitis 19 to 46 years old, who received SLIT for two years with standardized allergen extracts (Sevapharma) were studied. Group 1 included 30 patients receiving monotherapy with a mixture of grasses I or Artemisia. Group 2 included 30 patients, receiving combination therapy of a mixture of grasses I or Artemisia and mixture of house dust mites or indoor moulds. The control group included 30 healthy subjects. CD4+T-cells were assayed for intracellular cytokines after stimulation with PMA plus ionomycin. 

Results: CD4+T-cells (IL-4+ cells, IL-5+ cells, IL-13+ cells, or IL-17+ cells) were significantly increased before SLIT compared to controls but were reduced after the 2nd year of SLIT. IFN-γ+ cells increased after the 2nd year of SLIT. Comparing CD4+cells before and after SLIT in patients of Group 1, IL-4+ was reduced from 0.91 to 0.3; IL-17+ reduced from 0.75 to 0.38; IL-5+ reduced from 4.71 to 2.05; IL-13+ reduced from 4.9 to 1.58 and IFN-γ+ cells increased from 13.37 to 18.85. In Group 2, IL-4+ cells were reduced from 0.75 to 0.28; IL-17+ reduced from 0.5 to 0.2; IL-5+ reduced from 6.11 to 1.88; IL-13+ reduced from 4.95 to 2.06 and IFN-γ+ increased from 14.53 to 18.65.

Conclusions: Reduction of IL-4+, IL-17+, IL-5+ and IL-13+ CD4+T-cells and increase of IFN-γ+ CD4+T-cells demonstrated that SLIT can modulate immune responses in mono- and poly-sensitized patients.