Comparison of Different Protocols for the Induction of Experimental Allergic Rhinitis Mice
Monday, March 7, 2016
South Exhibit Hall H (Convention Center)
Alexander A. Babakhin, MD, PhD, A. A. Laskin, O. Y. Kamishnikov, Musa Khaitov, Lawrence M. DuBuske, MD, FAAAAI
Rationale: Experimental models of allergic rhinitis using different protocols were compared.

Methods: BALB/c mice group 1 were intraperitoneally (i.p.) sensitized with 25 µg/mouse of ovalbumin (OVA) together with Al(OH)3 three times in four week intervals and challenged by 12 intranasal applications (INAs) with 25 mg/ml OVA on consecutive days. Group 2 mice - same protocol with 50 mg/ml. Group 3 mice - OVA by INAs (12 days) with 25 mg/ml and in a two week interval with OVA by two series of INAs (25 mg/ml) during 12 days. Group 4 mice were sham sensitized (i.p.) and challenged by INAs with PBS. After the last challenge sneezing was counted for 5 min. Hyper-responsiveness to methacholine was measured and nasal tissues were removed for histology. Serum anti-OVA IgE, IgG1, IgG2a antibodies were detected by ELISA.

Results: Group 1 showed maximum sneezing. Highest anti-OVA IgE was observed in groups 1 and 2. Highest levels of anti-OVA IgG1 were observed after the third sensitization in group 3. The level of anti-OVA IgG1 was very high in groups 1, 2, 3. Anti-OVA IgG2a had significantly increase in Groups 1, 2, 3. AHR in groups 2 and 3 was significantly higher than group 4. Mild histological changes were found in group 3, moderate in group 1 and severe in group 2.

Conclusions: i.p allergen injections together with adjuvant and intranasal allergen challenge with 25 µg/mouse of allergen is the most appropriate for induction of mouse model of allergic rhinitis.