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An Exploratory Proof of Concept Study to Quantify the Major Cat Allergens, Fel d1 and Fel d4 from Domestic House Cats
Saturday, March 5, 2016
South Exhibit Hall H (Convention Center)
William H. Yang, MD, Suzanne Kelly, PhD, Nate Stepner, D.Litt, Douglas Boeckh, DVM, Jacob Karsh, MD, Jimmy Yang, MBA
Rationale: Cat dander is ubiquitous in our environment and is one of the most potent indoor allergens causing an IgE mediated Type 1 allergic response.  While patients may be sensitized to several different allergens found in the dander, the major ones are Fel d1 and Fel d4 with more than 80% of these individuals exhibiting IgE antibodies to these two allergens.  The purpose of this preliminary work was to measure the levels of Fel d1 and Fel d4 found in saliva, fur and urine of male and female domestic house cats and to determine whether there are differences in allergen levels dependent on breed, gender, sterilization status and age.  

Methods: Cats volunteered by owners from a local animal hospital were used for this study.  Owners signed an informed consent prior to any sample collection. Twenty cats were studied, five in each of four cohorts: neutered males, neutered females, females not in oestrus and males.  The breed, age, weight and health status of each cat was recorded. Commercially available ELISA kits were used to measure the allergen levels and a standard curve created from the Fel d1 and Fel d4 standards.

Results: Triplicate samples were analyzed to allow for a sufficient sample size for analysis and comparison. 

Conclusions: The information generated by this study will be used to determine the characteristics of cats to be housed in a cat allergen challenge room in order to obtain consistent levels of airborne allergen.