Methods: Patients within the Vanderbilt system with diagnosis of allergic bronchopulmonary mycosis were identified and recruited to the study. Peripheral blood mononuclear cells were isolated and placed under conditions to enhance B-cell growth in cell culture. B-cell cultures were screened for IgE secretion by ELISA and fused with human derived myeloma partners using electrical cytofusion methods. Human hybridomas were identified by placing in selective media (hypoxanthine, aminopterin, and thymidine) and subsequently assaying for IgE secretion.
Results: We report the production of IgE secreting human hybridomas from patients with allergic bronchpulmonary mycosis. Additional characterization of these antibodies will be presented.
Conclusions: Production of monoclonal IgE represents a novel tool to understand the evolution of the B-cell response in allergic patients.