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Induction of Tolerogenic Dendritic Cells Using Co-Culture with Human Olfactory Mucosa-Derived Mesenchymal Stem Cells
Sunday, March 6, 2016
South Exhibit Hall H (Convention Center)
Andrei Y. Hancharou, N. H. Antonevich, Lawrence M. DuBuske, MD, FAAAAI
Rationale: Human olfactory mucosa-derived mesenchymal stem cells (hOM-MSCs) may impact the immunophenotypic profile of human dendritic cells (DC).

Methods: hOM-MSCs were obtained from 6 patients with non-inflammatory nasal diseases. DC were obtained from monocytes using standard 6-day (GM-CSF/IL-4) protocol. hOM-MSCs were co-cultured with DC. DC were also cultured with LPS (positive control) and with hOM-MSCs conditioned media (CM). After 72 h of culture DC were assayed for immunogenic (CD80, CD86, HLA-DR) and tolerogenic markers (CD85k, CD273) as well as activation molecules (CD32 and CD83). 

Results: DC matured with LPS had increased  expression of CD32, CD80, CD83, CD86 and HLA-DR molecules. DC cultured with hOM-MSCs CM had slight increased expression of CD80 while maintaining immature phenotype. DC co-cultured with hOM-MSCs hads significantly increased expression of both immunogenic (CD86, CD32, p<0.05) and tolerogenic markers: CD85k (iDC – 50.2 (21.3-70.5)%, mscDC – 75.5 (31.2-86.4)%, p=0.03) and CD273 (iDC – 29.9 (24.3-37.0)%, mscDC – 40.6 (35.1-54.0)%, p=0.02).

Conclusions: DC cultured in the hOM-MSCs CM had immature phenotype. hOM-MSCs induce a tolerogenic profile in DC.