Expression Pattern of Peripheral Blood Mononuclear Leucocyte GABA Receptors and Calcium Signaling Genes
Sunday, March 6, 2016
South Exhibit Hall H (Convention Center)
Leonid P. Titov, MD, PhD, A. B. Kapitau, K. I. Pavlov, I. M. Goloenko, Lawrence M. DuBuske, MD, FAAAAI
Rationale: Neuromediators including gamma-aminobutyric acid (GABA) have immune modulation, impacting airway epithelium of allergic asthma stimulating mucus production. 

Methods: PBML gene expression profiling was performed using Human Discover Chips™ and Neurotransmitter Receptors and Regulators Arrayit Pathways™ Focused microarrays (Arrayit corporation, California, USA). GABA A2 receptor polymorphic locus rs279826 analysis was performed using standard PCR with subsequent Mnl1 endonuclease restriction. 

Results: Microarray methodology demonstrated significant expression levels in PBML for calcium-associated ion channels and second messengers: chloride channel 1, calcium activated gene, calmodulin 1 gene, S100 calcium-binding protein A7 gene, vacuolar ATFase gene (increasing level of expression: CLCA1 < CALM1 < S100A7 < VPS33B) with slight expression of cholinergic receptors and nicotinic genes compared with intensive expression of gamma-aminobutyric acid (GABA) A receptor alpha 2 gene. 

Conclusions: GABA A2 receptors have high expression in PBML. PBML GABA receptors and calcium associated second messenger expression indicate a significant peripheral effect of neurotransmitters.