Methods: A comprehensive evaluation of semen quality was done on aliquots of ejaculates which were analyzed for Hsp60, IL-8, IFN-γ, IFN-α in infertile patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Microscopy semen was analyzed to measure sperm concentration and percentage of motility using WHO criteria.
Results: Нsp60 was present in a soluble form in 100% semen in men with CP/CPP (n=89). The presence of Hsp60 in semen correlated with the concentration of IL-8 (p<0.01, r=0.273), and the number of leukocytes (p<0.05, r=0.254). Hsp60 was not related to semen quality under microscopic analysis. Hsp60 did not correlate with IFN-γ or IFN-α levels (p>0.05).
Conclusions: While IL-8 correlated with Hsp60 in seminal fluid, Hsp60 failed to be associated with semen quality in infertile patients with chronic prostatitis/chronic pelvic pain syndrome.