The home bacterial community of allergic and asthmatic individuals has been a growing area of interest in the field of respiratory disease.
Dust DNA samples (n=21) were taken from homes in the Healthy Homes Project. DNA was isolated using the MoBio-PowerLyzer-PowerSoil DNA isolation kit and bacterial 16s rDNA was amplified with universal primers for V3 and V4 hypervariable regions. Multiplexed 16S ampliconTruSeq™ sequencing was performed on an Illumina HiSeq1500. Paired end reads were demultiplexed and analyzed using QIIME. The bacterial microbiome of non-smoking homes (n=13) was compared to smoking homes (n=8). Similarly, the microbiome of homes without pets (n=11) was compared to homes with pets (n=10).
21 dust samples received a total of 104,760 counts across 1,042 operational taxonomic units. Common bacteria phyla included Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. Higher levels of Proteobacteria and Bacteroidetes were observed in the smoking group and the pet group. The smoking group also had higher levels of Firmicutes. More of the family Moraxellaceae was observed in smoking homes, and more of the family Enterobacteriaceae was observed in non-smoking homes. On average, the non-smoking group received more counts and a greater diversity of families and genera than the smoking group. The non-pet group also received more counts and a greater diversity than the pet group. Of the top 10 families and genera, the top nine belonged to phylum Proteobacteria with the tenth belonging to Actinobacteria.
These results suggest that smokers’ and pet owners’ microbial biome is not as abundant and less diverse.