572
Latino Patients With Chronic Rhinosinusitis (CRS) Have a Distinct Nasal Microbiome Associated with Poor Disease Outcome
Sunday, March 5, 2017: 3:00 PM
Rooms B312-B313 (Georgia World Congress Center, Building B)
Sherlyana Surja, MD, , , , , ,
Rationale: Previous study has shown that African American (AA) and Latinos with chronic rhinosinusitis (CRS) have more severe disease with worse outcome. Changes in the nasal microbiome were believed to influence mucosal inflammation in CRS. We aimed to study whether race and ethnicity is associated with differences of the nasal microbiome in CRS patients

Methods: A nasal cotton swab was collected under endoscopic guide from patients with CRS and healthy controls. We assessed nasal microbiota composition based on 16S-ribosomal-RNA gene sequencing from phylum to genus level. Subsequent bioinformatics analyses were used to test differences in the nasal microbiota composition and identify key taxa which were most strongly altered in different racial groups of CRS.

Results: Twenty-five nasal samples were analyzed; 8 were AA, 8 were Mexican-Latinos and 9 were non-Latino Whites. At phylum level, Latino CRS cases had increased Actinobacteria compared with AA and Whites. At genus level, Corynebacterium was significantly increased in Latino patients compared with other groups (mean Operational Taxonomic Units[OUT] were 1647.6, 129.9 and 206.5 in Latino, AA and whites respectively,p<0.05). Corynebacterium was also increased in refractory CRS cases that needed repeat surgical intervention (mean OTU of 912.5) compared to controls (mean OTU of 102.5), p<0.05 and CRS cases responsive to medical therapy (mean OTU of 11.1),p<0.05.

Conclusions: We found that Latino CRS patients have significantly increased Corynebacterium in comparison with other races in their nasal microbiome. Corynebacterium was independently associated with refractory CRS. Future studies are needed to investigate the mechanism of changes in nasal microbiome in these Latino patients.