391:
Immunological changes induced by Pru p 3-glycodendrimers
Sunday, March 4, 2018
South Hall A2 (Convention Center)
Maria Francisca Palomares, PhD, Francisca Gomez, MD, PhD, Ainhoa Mascaraque, PhD, Gador Bogas, MD, PhD, Javier Ramos-Soriano, BsC, Maria José Rodríguez, BSc, Maria Jose Torres Jaén, MD, PhD, Javier Rojo, PhD, Cristobalina Mayorga, PhD
RATIONALE:

Glycodendrimers functionalized with mannose are promising structures to enhance allergen interaction with antigen presenting cells via C-type lectin receptors and modulate the immune response. They can present multiple peptide epitopes in an allergen specific manner, with potential applications for designing vaccines for food allergy. We analysed the in vitro immune response induced by Pru p 3-mono or -tetravalent glycodendrimers with different bonds, in peach allergic patients.

METHODS:

Pru p 3-mono (D1) and tetravalent (D4) structures were functionalized with mannose with different bonds, D1-M or D4-M (ester bond) and D1-M-o- or D4-M-o- (ether bond). Responses were analysed in peach allergic patients and healthy controls, by studying monocyte-derived-dendritic cell (moDC) maturation and interaction using blocking DC-Sign (αCD209) and mannose (αCD206) receptors, and lymphocyte proliferation, by flow cytometry.

RESULTS:

The glycodendrimers with an ester bond, D1-M and D4-M, induce moDC maturation in both patients and controls. T cell proliferation, mainly CD8, is higher with the tetravalent structures in peach allergic patients. Changes were less marked for glycodendrimers with an ether bond. The interaction of glycodendrimers with moDCs showed that αCD209 produced a significant reduction of D1-M and D4-M induced-moDC maturation. D1-M-o or D4-M-o- in presence of the αCD209 and αCD206 reduced moDC maturation. These changes were correlated with the inhibition of lymphocyte proliferation in peach allergic patients.

CONCLUSIONS:

The nature of the glycondendrimer structure influences the immune response. The glycondendrimers with an ether bond led to diminished moDC maturation and T cell proliferation, suggesting they could be candidates for food allergy vaccine development.