METHODS: We performed retrospective analysis of 55 patients who were suspected of fish allergy. Diagnosis of allergy to each kind of fish was made by positive OFC or immediate induced symptoms after eating a specific fish. Negative fish allergy was based on negative OFC or regular eating of a specific fish. Whole blood was incubated with various kinds of fish extracts and induced expression CD203c on basophils was analyzed by a flow cytometry. ImmunoCAP sIgE to each kind of fish was also measured. Predictive performance of the two tests for fish allergy was evaluated using receiver-operating characteristic (ROC) analysis.
RESULTS: Area under the curve (AUC) for BAT was higher than sIgE; tuna: 0.94 vs. 0.62, salmon: 0.90 vs. 0.74, mackerel: 0.90 vs. 0.69, respectively. AUC for BAT in diagnosis of flounder, red snapper, yellowtail and bonito allergies, for which sIgE test was not available, was also high at 0.91, 0.87, 0.81 and 0.80, respectively.
CONCLUSIONS: BAT using CD203c expression may a reliable method to predict fish allergy.