883:
Effect of adipose-derived stem cell and non-methylated CpG-ODN on Peripheral blood CD4+CD25+regulatory T cell in young mice of food allergy
Monday, March 5, 2018
South Hall A2 (Convention Center)
Chengzhong Zheng Sr.
RATIONALE: To observe the expression level of peripheral blood CD4+CD25+regulatory T cell in young mice of food allergy treated with adipose-derived stem cell and non-methylated CpG-ODN,and to explore the immune intervention effect.

METHODS: Forty female BALB/c mice (2-3 weeks old) were randomly divided into control, allergic model, ADSC and CpG-ODN treatment groups(n=10 each). A young mouse model of food allergy was established by OVA sensitization.The mice in ADSC group were intraperitoneally injected with 1×106ADSCs on days 15 and 30.The mice in CpG-ODN treatment group were intraperitoneally injected with CpG-ODN solution 1 hour before every OVA provocation sensitization.The OVA-IgE of peripheral blood of all mice were detected by ELISA. The levels of CD4+CD25+regulatory T cell in peripheral blood of all mice were detected by flow cytometer.

RESULTS: The expression levels of OVA-IgE were lower in the ADSC group and the CpG-ODN group than in allergic model (bothP<0.05).They were higher in ADSC group and CpG-ODN group than in allergic model (P<0.05).

CONCLUSIONS: ADSC and non-methylated CpG-ODN can increase the expression levels of CD4+CD25+regulatory T cell in peripheral blood and decrease the content of specific IgE (OVA-IgE). They could play a certain role in inducing immune tolerance in food allergy.