Genetic variants of VDR and vitamin D insufficiency are associated with chronic spontaneous urticaria in Colombian adults.
Saturday, March 3, 2018
South Hall A2 (Convention Center)
Eduardo Egea, MD, PhD, Luis Fang, DDS, MSc., Nicole Pereira-Sanandres, Fernando R De la cruz-Lopez, Sofia Moreno-Woo, Gloria Garavito-De Egea, MD, PhD
RATIONALE: chronic spontaneous urticaria (CSU) is a complex skin disease of unclear etiology. Several studies have demonstrated how serum vitamin D deficiency and SNPs polymorphism in the receptor of vitamin D gene (VDR) influence diverse pathologies with immune compliance. This study analyzed the association between polymorphisms of VDR SNPs and serum levels of vitamin D (VitD) with CSU in a Caribbean multi-ethnic adult patients sample.

METHODS: an analytical case-control study was carried out with 150 non-family related subjects: 50 CSU patients and 100 healthy controls matched by age. All of them settled in the Colombian Caribbean area. The TaqI [rs731236 A/G], ApaI [rs7975232 A/C], FokI [rs2228570 A/G] and BsmI [rs1544410 C/T] polymorphisms were genotyping by qPCR. Serum levels of VitD (normal values considered for this study - IOM: 30-100ng/ml) were quantified by EIA. p-values <0.05 was considered statistically significant.

RESULTS: the G allele of TaqI and the A allele of FokI were shown as risk factors for CSU; OR:3.25 (95%CI: 1.9 to 5.4; p:0.00) and OR:1.81 (95%CI: 1.09 to 3.02; p:0.013); respectively. The haplotypes ACCG, AACG, GACG, GCCA showed statistical differences in their distributions between cases and controls (p<0.05). Eighty percent (n=40) of the cases showed VitD insufficiency unlike controls (19%; n=19) (p: 0.000). Analysis of the data was consistent that the VitD insufficiency is a risk factor for CSU OR:17.9 (95%CI: 7.3 to 43.5; p:0.00).

CONCLUSIONS: our results showed a association of VDR-SNPs polymorphisms and VitD insufficient levels with CSU in this sample studied. These findings should be reproduced in other multi-ethnic groups.