Cell Surface Markers and Immunogenetic Characteristics of Tumor Cells Suitable for Preparing Personalized Antitumor Vaccine against Solid Tumors
Sunday, March 4, 2018
South Hall A2 (Convention Center)
Tatiana A Slavyanskaya, 85478, Svetlana V Salnikova
RATIONALE: The aim of this study was to determine the surface markers of cells and immunogenetic characteristics of urothelial carcinoma cells (UCC).

METHODS: Operative material from 54 patients with muscular invasive and non-invasive forms of UC out of which only 10 were consistently transferred for creation of cellular tumorous cultures, were used and researched before 10 (early) and over 30 (late) passages. Preparations were analyzed with consideration for international classification of chromosomes (Ch). Number of analyzed cells depended on the quantity of identified clones and lied within the range from 20 to 100. Tumor-specific cancer-testis antigens (CTA) expression was evaluated by flow cytometry. Mathematical processing was performed with the use of SPSS 23.0

RESULTS: It was established, that UCC had cytogenetic changes: deletion of 9 Ch (66.7%), absence of Y Ch (50%) and monosomy of 13 Ch (33.3%). In rare cases changes in 1,3,7 Ch, monosomy of 17 Ch, trisomy of 7 Ch were detected. In case of LP of UCC, significant increase of the number of genetic changes in a form of division of previously homogeneous population into subclones differing in ploidy (up to 56 Ch) and quantity of changed Ch is observed. After comparison of the results with data, obtained by flow cytometry method, certain correlation of these changes to decrease of CTA expression (GAGE, BAGE, MAGE и NY-ESO-1) (p<0.05) was established.

CONCLUSIONS: There should be used autologous UCC in early or allogenic UCC marked with a cytogenetic consistency and stable expression of CTA when creating of PDAV against UC.