METHODS: Using mice sensitized and challenged with OVA, as well as mice sensitized and challenged with saline, we investigated whether nectin-4, and Src/Rac/JNK pathway be involved in the pathogenesis of bronchial asthma by western blotting and Immunohistochemical staining. Moreover, we also checked relationship between the levels of nectin-4 levels in blood from asthmatic patients and clinical variables.
RESULTS: Nectin-4/afadin in lung tissue was significantly increased in OVA/OVA mice compared with control mice. Src/Rac/JNK pathways were involved in OVA/OVA mice. The plasma nectin-4 levels were significantly decreased in non-treated asthmatic patients compared to those of control subjects, and anti-asthmatic treatment were recovered to normal levels. The plasma nectin-4 level was correlated with FEV1 and methacholine PC20.
CONCLUSIONS: These findings thus raise the possibility that nectin 4 is involved in asthma pathogenesis.