803:
Effect of anti-IgE antibody on anaphylaxis in a mouse model of wheat-gliadin allergy
Monday, March 5, 2018
South Hall A2 (Convention Center)
Hajime Arifuku, Kumiya Sugiyama, Shingo Tokita, Hirokuni Hirata, Yasutsugu Fukushima
RATIONALE: To find a novel treatment for anaphylaxis due to wheat-gliadin allergy, we evaluated the effect of anti-IgE antibody (IgE Ab) on anaphylaxis in a mouse model of wheat-gliadin allergy.

METHODS: B.10A mice were injected with gliadin intraperitoneally and subcutaneously to sensitize them to gliadin. The mice were challenged with gliadin 24 h after IgE Ab injection. Three treatment groups were analyzed. The first group was sensitized to gliadin and treated with IgE Ab (gliadin IgE Ab group), the second was sensitized to gliadin but not treated with IgE Ab (gliadin group), and the third was not sensitized and not treated (control group).

RESULTS: Gliadin IgE levels were significantly higher in the gliadin-sensitized groups (optical density [OD] = 0.451) than in the control group (OD = 0.121, p < 0.01). Serum histamine levels were significantly lower in the gliadin IgE Ab group (32.6 ng/mL) than in the gliadin group (57.8 ng/mL, p < 0.01), and similar to that in the control group (39.9 ng/mL). The body temperature decrease after gliadin challenge was significantly lower in the gliadin IgE Ab group (1.0 °C) than in the gliadin group (1.9 °C, p = 0.07), and similar to that in the control group (1.1 °C).

CONCLUSIONS: IgE Ab inhibits anaphylaxis due to wheat-gliadin allergy in this mouse model. In ongoing studies, we are evaluating other mediators of anaphylaxis. We will present our final results at the 2018 AAAAI/WAO Joint Congress.