Cryj 1, a major allergen of Japanese cedar pollen, up-regulates the effector functions of eosinophils
Saturday, March 3, 2018
South Hall A2 (Convention Center)
Takehito Kobayashi, MD, PhD, Kazuyuki Nakagome, MD PhD, Sachiko Miyauchi, MD, Toru Noguchi, MD, PhD, Kouhei Saitou, MD, Tomoyuki Soma, MD PhD, Hidetomo Nakamoto, MD, PhD, Makoto Nagata, MD PhD
RATIONALE: Japanese cedar pollinosis is the most common form of pollinosis in Japan and has recently remarkably increased. There is evidence that eosinophils play important roles in the development of aggravating the clinical symptoms of the allergic rhinitis or bronchial asthma. In this study, we examined whether Cryj 1, which is the major Japanese cedar pollen allergen, could directly modifies eosinophil functions such as superoxide anion (O2) generation and adhesion.

METHODS: Eosinophils were isolated from the blood of healthy volunteers and subsequently stimulated with either Japanese cedar pollen (crude) or Cryj1 (the major Japanese cedar pollen allergens), and their adhesion to recombinant human intercellular cell adhesion molecule (rh-ICAM)-1 was measured using eosinophil peroxidase assays. The generation of eosinophil superoxide anion (O2) was examined based on the superoxide dismutase-inhibitable reduction of cytochrome C.

RESULTS: Both the crude extract of Japanese cedar pollen and Cryj1 directly enhanced the adhesion of eosinophils to ICAM-1 (N=6 each, both P<0.01). Furthermore, the crude Japanese cedar pollen extract and Cryj1 also significantly induced eosinophil O2generation (N=6 each, both P<0.01).

CONCLUSIONS: These findings suggested that Japanese cedar or Cryj1 directly up-regulates eosinophil functions such as O2 generation and cell adhesion. These effects may be involved in the activation of eosinophils and could possibly aggravate the airway diseases associated with Japaneses cedar pollinosis.