487:
Diagnostic value of basophil activation test on wheat-dependent exercise-induced anaphylaxis(WDEIA)
Sunday, March 4, 2018
South Hall A2 (Convention Center)
Qian Zhang
RATIONALE:

we will evaluate the diagnostic value of basophil activation test(BAT) on wheat-dependent exercise-induced anaphylaxis(WDEIA), and analyze whether BAT can effectively assess the risk and severity of anaphylaxis to reduce the need for oral food challenge test.

METHODS:

1. Serum sIgE of wheat and its components were measured. BAT was carried out by flow cytometry, using wheat water / salt-soluble protein, glutenin and gliadin as stimulants. The clinical of WDEIA were used as diagnostic criteria, and the ROC curve was used to compare the value of BAT and sIgE in the diagnosis of WDEIA.

2. According to the different clinical manifestations, the severity of anaphylaxis was divided into four grades , the best wheat protein concentration was used in BAT test and the severity of WDEIA symptoms were analyzed.

RESULTS:

1. The area under the ROC curve of the BAT test was significantly larger than that of sIgE (P <0.001). The area under the ROC curve (0.961, P <0.001) of the BAT test of the gliadin as the stimulus was greater than that of other components as the stimulus, sensitivity is 92.5% and specificity is 88.9%.

2. Spearman correlation analysis showed that the proportion of activated basophils was significantly correlated with the severity of the disease (r = 0.652 and 0.798, P <0.01) when the glutenin and gliadin were used as stimulators in the BAT test.Wheat glutenin sIgE (f79) was associated with disease severity (r = 0.358, p = 0.023).

CONCLUSIONS:

BAT is the best indicator for diagnosing and predicting the severity of WDEIA.