Investigation of candidate proteins associated with allergic march in the Der p-induced experimental murine model
Sunday, March 4, 2018
South Hall A2 (Convention Center)
Woo-Sung Chang, PhD, Kyu-Tae Jeong, Ji-Hye Do, Sung-Hee Lee, Gyo Jeong Gu, Jeom-Kyu Lee, PhD
RATIONALE: The “Allergic march” is characterized by the progression of allergic diseases that atopic dermatitis is developed in infants, followed by allergic asthma and allergic rhinitis in children. Several studies for the allergic march have been reported, but our understanding of what factors are involved in its mechanism remains limited. In this study, we investigated that there were candidate proteins showing specific expression patterns in the murine model.

METHODS: Atopic dermatitis was induced by repeated Dermatophagoides pteronissunus (Der p) exposure with 1,2-Dinitrochlorobenzene (DNCB) on ear skin, followed by asthma development by Der p sensitization and repeated intranasal challenge. After last challenge, we investigated IgE, Th2 cytokines, cell recruitment and airway hyperresponsiveness to confirm the development of allergic march. Two-dimensional electrophoresis and mass spectrometry was performed to investigate allergic march-related proteins.

RESULTS: In the allergic march-induced group, the expression level of IgE and Th2 cytokines were higher than the asthma only induced group as well as normal control. Airway hyperresponsiveness and cell infiltration including eosinophils were also significantly aggravated. We found more than fifty spots from bronchoalveolar lavage fluid (BALF) that showed different expression pattern in 2-D gel among the groups, and then eight candidate proteins were finally identified by MALDI-TOF/TOF analysis.

CONCLUSIONS: We found eight candidate proteins in our experimental allergic march model, and examined their features including the association with allergic responses. Some of them were thought to have the potential to be allergic march-related factors, but further studies to reveal their distinct roles in the progression of allergic march are needed.