722:
Peculiarities of phenotyping of lymphocytes in patients with pollen allergy against the backdrop of active herpesvirus infection type 4, 5 and 6
Monday, March 5, 2018
South Hall A2 (Convention Center)
Valentyna Chopyak, Svitlana Zubchenko, Sergey Yuriev, Olena Gubska
RATIONALE: Study the peculiarities of the phenotypic characteristics of lymphocytes and their activation markers in patients with pollen allergy against herpesvirus infections.

METHODS: 162 persons were examined with clinical and laboratory manifestations of pollen allergy, age 32,6 ± 2,4 years, 53,1% - women, 46,9% - men. SPT Diater, Spain, total and specific IgE, ELISA. EBV, CMV, HHV6 by the polymerase chain reaction using Rotor Geen 6000. Phenotyping of lymphocytes - a flow cytofluorimetre “Bekton Dickenson” (USA).

RESULTS: Based on specific allergic studies, pollen allergy was confirmed in 158 (97.5%) patients, in 112 (70.8%) of whom polysensitization was detected, and in 46 (29.2%) monosensitization was confirmed. According to the data of molecular genetic studies, in 128 (81.0%) cases activated herpesvirus infection was detected: in 48 (37.5%) cases - monoinfection, in 80 (62.5%) - combined infection and in most 47 (58.7%) cases EBV + HHV6. Patients were divided into 3 groups: the first one - people with pollen allergy without viral activity; the second one - people with pollen allergy + viral activity. Third group - 50 healthy people. Patients in the second group showed an increase in CDHLA-DR + cells and B-lymphocytes and decreased NK-cells (p<0.05). In 75.0% of these patients, increases of levels of total IgE, that was 1.1 times more than in patients in the first group.

CONCLUSIONS: In patients with pollen allergy against the backdrop of active herpesvirus infection, there was an increase in the cell-dependent inflammatory process with the creation of conditions for the formation of hyper-IgE syndrome.