Peach pollen has been shown to be relevant in the induction of sensitisation in areas of rosaceae fruit agriculture. We have identified a protein of 15 kD as Pru p X that is recognized by patients who are sensitized to peach pollen. Our aim was to prove that in cases with skin test positive to peach pollen and Pru p X, symptoms were induced after nasal challenge with this new allergen.
We evaluated subjects who referred nasal/conjunctival symptoms during the exposure to peach tree pollen. Criteria for inclusion were skin prick test positive to peach pollen extract as well as to Pru p X. A nasal provocation tests was made with whole pollen peach extract (5 mg/ml) and if positive further provocation with Pru p X (20 mcg/ml)was made. Response was measured by acoustic rhinomanometry and symptoms score.
Out of 8 cases evaluated with the selection criteria mentioned above, 5 cases we showed a positive nasal response to both whole pollen extract and Pru p X. In all of them there was a decrease in acoustic rhinomanometry greater than the 20% of nasal volume plus the appearance of symptoms within the first 30 minutes after challenge and persisted for several hours.
Pru p X is a relevant allergen present in peach pollen that induces symptoms after pollen exposure and challenge with the purified Pru p 3. Futher work in in progress to ass the clinical relevance.