Low Density Lipoprotein enhances the in vitro production of IL-18 from periferal blood mononuclear Cells
Monday, March 5, 2018
South Hall A2 (Convention Center)
Ammar K. Daoud, MD FAAAAI, Nesreen A. Saada, Nizar M. Abu Harfiel, Nawar A. M. Eisa
RATIONALE: We wanted to study the effects of in vitro hyperlipidemia (high Low Density Lipoprotein (LDL) or Bad Cholesterol) on one aspect of the immune function as of level of cytokine production of IL-18 (related to the IL-1 family)

METHODS: After getting the apporvals of our IRB's and from concenting patients (Uncontrolled Diabetes Mellitus and Hyperlipidemia) Peripheral Blood Mononuclear Cells were isolated from 10 diabetic and 5 health controls. Cells were incubated in RPMI-1640 culture media with 200 mg/dL glucose, 1 μg/mL LPS and with 0, 130 and 260 mg/dL LDL for 22 hours. IL-18 levels were measured using ELISA kits (DueSet ELISA kits, R&D, UK) read at 450 nm wavelength. Mann-Whitney U test and Wilcoxon signed rank tests were used to determine the statistical significance of the differences and p values less than 0.05 to be positive.

RESULTS: Mean IL-18 produced at 0 mg/dL of LDL was 127.8 pg/ml (±44.2), at 130 mg/dL of LDL was 160.4 pg/ml (±53.9) and at 260 mg/dL LDL was 168.5 pg/mL (±30.1) with p value of .017 * significant

CONCLUSIONS: Hypercholestreolemia is part of the Metabolic Syndrome is associated with chronic inflammatory processes in the body one one hand, can be associated with abormal or exaggerated immune responses like excessive IL-18 production.