Allergic airway diseases are characterized by the presence of specific IgE antibodies directed to the inhalant allergens, including those derived from the house dust mites (HDM), such as the Dermatophagoides pteronyssinus group 1 (Der p 1) and group 2 (Der p 2) allergens. Mite allergen crude extracts are currently used throughout the world for both diagnostic and specific immunotherapy procedures. The definition of patterns of sensitized patients from different geographical areas to the allergenic source is quite relevant to fulfill the established criteria for the manufacturing of allergenic extracts.
We selected 21 non-consecutive patients sensitized to Dermatophagoides pteronyssinus with persistent moderate to severe rhinitis according to the ARIA Guidelines. Skin prick test (SPT) with standardized extracts of Dermatophagoides pteronyssinus. Serum blood samples were obtained from all participating subjects. Total IgE, specific IgE to Dermatophagoides Pteronyssinus, Der p 1 and Der p 2. Protein allergenic characterization (SDS PAGE and Western Blot) was performed in all 21 serum samples.
Nineteen out of 21 subjects (14 females, 11 to 46 y.o.) showed a positive SPT to both Dermatophagoides pteronyssinus. The patterns of sensitization to Dermatophagoides pteronyssinus were obtained and the levels of specific IgE to Der p 2 were higher than Der p 1 (40.49 kU/L; 27.47 kU/L)
CONCLUSIONS: Group 2 (Der p 2) of Dermatophagoides pteronyssinus was the most recognized allergen among the moderate-severe persistent allergic rhinitis selected population, therefore it is necessary to bear in mind according to establish the properties of allergenic extracts.