METHODS: The chamber, volume 14.7 m3 was designed and built to accommodate two neutered cats and 1-2 subjects. Samples were obtained at 3 locations in the chamber using portable air samplers (Gillian 5000) with glass fiber filters (Millipore), flow rate 4 L/min. Feld1 was quantified using ELISA (Indoor Biotechnologies). Over 20 weeks, 30-minute air samples and floor and wall swabs were collected to measure Feld1. Fans and blanket shaking were evaluated as means to aerosolize dander.
RESULTS: With no disturbance, air Feld1 levels were mostly below the limit of detection in the 20 weeks following the cats’ introduction to the room. Over the same time, wall (1.7 to 19.2 ng/m2) and floor Feld1 (15.1 to 138.2 ng/m2) increased. Fans placed 1.5 and 7 feet above the floor aerosolized Feld1 (12.6 ± 19.8 and 9.17 ± 6.1 ng/m3, respectively). Shaking the cats’ blankets generated 45.9 ± 60.1 ng/m3 in the air and shaking every 15 minutes 111.7 ± 46.3 ng/m3.
CONCLUSIONS: Feld1 increased on walls and floor over 20 weeks of cat residence but required intervention for aerosolization. Blanket shaking generated higher levels of Feld1 in the air.