AIM : To validate Strasbourg EEC by determining the concentration of Fel d1 inducing 50% of early asthmatic response (EAR) and/or late phase asthmatic response (LAR) in subjects sensitized to cat.
METHODS: It was a randomized, double blind, cross-over study including group A:20 asthmatic subjects allergic to cat and group B:10 asthmatic subjects allergic to another allergen. All subjects were first exposed to placebo. Group A was exposed to 2 Fel d1 concentrations. The number and size of particles were recorded online during the exposure. Group B was exposed to the concentration of Fel d1 which fulfills the objective of the study.
RESULTS: The mean age of subjects was 29 years (±8). For the 2 concentrations of Fel d1 we obtained more than 50% EAR and/or LAR. The mean time necessary to obtain an EAR was: 59.7±8 min and 138.6±90 min for the LAR. The mean fall in FEV1 during EAR and LAR was -29,22% and -17,64% respectively. We didn't observe any severe reaction. No subjects in group B experienced any symptoms during exposure.
CONCLUSIONS: We have validated ALYATEC’s EEC in subjects with asthma sensitized to cat. We also demonstrated its specificity. That is of interest for future clinical studies with asthmatic treatments