222:
Effects of topical in vivo treatment on ovalbumin antibody production in mice
Saturday, March 3, 2018
South Hall A2 (Convention Center)
Greg A. Plunkett, PhD, Henry J. Legere, MD FAAAAI, Dennis Harris, Peter Hurwitz, President, Jose Toscano, James Strader
RATIONALE: Management of allergic rhinitis includes avoiding the exposure of allergens combined with drug therapy. For the patients that do not have substantial relief in allergic rhinitis symptoms or incur side effects, subcutaneous and sublingual immunotherapy can be a second line of treatment. This study looked at an alternative topical route of immunotherapy administration of allergen through the skin which may be beneficial in treating allergic rhinitis. IgE, IgG and lymph node T cell responses in mice were studied using ovalbumin antigen mixed with topical cream formulations with potential adjuvant properties applied to the skin.METHODS: BALB/c mice were immunized with ovalbumin mixed with alum to induce an IgE response. After 14 days ovalbumin mixes with topical creams and controls were applied to the back skin of the mice and IgE, IgG2a and T Cell biomarkers from draining lymph nodes were measured up to 71 days and results compared with intraperitoneal injection controls.RESULTS: Mice developed IgE to ovalbumin after 14 days and application of cream formulations showed a dose dependent reduction in IgE compared to controls at 42 and 71 days. Topically applied ovalbumin showed an increase in sIgG2 comparable to intraperitoneal injection in some formulations and biomarkers such as CD69 indicated that the antigen reached draining lymph nodes.CONCLUSIONS: Initial results from this mouse study demonstrated that topical administration of allergens mixed in creams can have an immune response and may provide an alternative route for immunotherapy.