Correlation of Exhaled Nitric Oxide Levels with Salivary Melatonin Levels in Adults with and without Allergy/Asthma
Sunday, March 4, 2018
South Hall A2 (Convention Center)
Tehila A Saadia, MD, Andrew Hasenzahl, BS, MPH, Maja Nowakowski, PhD, Emmanuel Kwesiga, MBChB, Yi-Feng Chen, MD, Rauno Joks, MD

We have previously reported that longer duration of sleep is associated wtih lower AM FeNO levels. As melatonin, a photoneurohormone which regulates sleep-wake cycles, can have anti-inflammatory effects, we sought to determine whether salivary melatonin levels correlate with FeNO.


IgE+adults with allergy/asthma (n=10, IgE:554.7 IU/ml ±541.7) and IgE- nonallergic adults (n=10, IgE:48.8 IU/ml ±38.4) had FeNO levels measured and salivary samples obtained for melatonin levels (Niox Vero, Circassia; EIA, Salimetrics, State College, PA) at 9-10 AM (> 1hr post meal) and 6 hrs later. Saliva was collected by unstimulated passive drool technique and frozen at -20C until assay. Salivary melatonin levels (pg/ml) were determined with a competitive immunoassay using HRP and tetramethylbenzidine. Pearson correlation coefficients and T-test were used in analysis.


There was no intraday variability in FeNO levels for either the IgE- (AM: 16.2 ppb ±7.5, PM: 15.8 ppb ±8.0) or IgE+(AM: 40.7 ppb ±40.8, PM: 33.5 ppb ±27.3) group (p=ns). While there was no significant change in melatonin from AM to PM for the IgE+ group (AM: 6.8 pg/ml ±8.2, PM: 18.1 pg/ml ±34.2)(p=0.3), there was a significant increase in salivary melatonin for the

IgE- group (AM: 6.5 pg/ml ±9.8, PM: 31.6 pg/ml ±26.52)(p=0.02). There was no correlation between FeNO and salivary melatonin level for either group, in either AM or PM (p=ns)

CONCLUSIONS: Daytime FeNO levels in IgE+ and IgE- adults are independent of circadian effects on salivary melatonin levels.