442:
IgE sensitization profiles of adult patients with moderate and severe atopic dermatitis: is there a role for molecular-based allergy diagnosis?
Sunday, March 4, 2018
South Hall A2 (Convention Center)
Karine Boufleur, MD, Janaina ML Melo, MD, PhD, Renata Nahas Cardili, MD, PhD, Marcelo ABR Amaral, Mariana PL Ferriani, MD, PhD, Thais N Mendonça, MD, Luana SM Maia, LuisaKarla P Arruda, MD PhD FAAAAI, Adriana S Moreno, PhD
RATIONALE: Atopic dermatitis (AD) is a chronic disease characterized by itchy inflamed skin. We investigated the profile of allergen sensitization in adult patients with AD using multiplexed allergen microarray.

METHODS: 39 patients with moderate and severe AD, attending the Allergy or Dermatology Clinics at our Institution, were enrolled. Total and specific IgE were quantitated by ImmunoCAP and ImmunoCAP-ISAC®, respectively.

RESULTS: Patients were aged 14-66 years-old (mean ± SD 34.3 ± 2.1 years), 64% female. Mean SCORAD was 36.6(2-90). Geometric mean (GM) of total IgE was 1,963 UI/mL (24-63,000UI/mL). Allergens from dust mites were dominant, with sensitization to Derp1, and to Derf1, Derp2, and Derf2 in 82% and 85% patients, with GM 27.6;50.0;39.2;45.4 ISU-E, respectively, followed by cat (38%), dog (36%) and grass pollen (36%) allergens. IgE to allergens from cockroach, fungi, most tree and weed pollens, latex and insect venoms was found in <20% of patients at low levels. Sensitization to walnut (33%), shrimp (31%), olive (28%) and peach (26%) allergens was most prevalent among foods, whereas IgE reactivity to milk and egg was present in 10% or less of the patients, with low levels of IgE antibodies in most cases. Only 6 patients (15.4%) presented IgE to the pan-allergen tropomyosin, and 3/39 (7.7%) were negative for IgE to all 112 allergens tested, with total IgE levels of 24, 38.7 and 156UI/mL.

CONCLUSIONS: IgE sensitization profiles of Brazilian adult patients with AD differed from those among patients with asthma, with less sensitization to cockroach and to tropomyosin, and from those among children with AD.