295:
Cockroach allergen induces atopic dermatitis and up regulates DCIR expression in mast cell
Saturday, March 3, 2018: 2:45 PM
S220EF (Convention Center)
Huang Yang, , ,
RATIONALE:

Large body of literatures have pointed toward mast cells as the driver of anaphylaxis, acute allergic reactions, and long-term pathophysiological changes that are associated with allergic inflammation. Dendritic cell immunoreceptor (DCIR) is Ca2+-dependent and has been shown to be expressed on antigen presenting cells and granulocytes. We hypothesize that DCIR plays a role in atopic dermatitis (AD).

METHODS:

Cockroach allergen was used to establish an AD mouse model and the role of mast cell and DCIR was examined. The role of DCIR in allergen uptake and mast cell activation was studied human mast cell line (LAD2).

RESULTS:

Cockroach allergen (CRE) exposure was able to induce characteristics of AD including higher eczema area and severity index (EASI) score, increased serum specific IgE with hyperplasia of the epidermis, increased dermal infiltrate and mild spongiosis upon histologic examination compared to mock mice. Furthermore, CRE exposure resulted in higher recruitment of mast cell to skin lesion with increased expression of DCIR. Interestingly, DCIR expression was abolished when the same AD model was created in the mast cell deleted (cKitW-sh/W-sh) mice. Furthermore, CRE-treated LAD2 cell up regulates DCIR expression, with higher CRE uptake, and secretion of β-hexosaminidase and IL-13. Lastly, DCIR was able to bind CRE using solid phase binding assay.

CONCLUSIONS:

Cockroach allergen can induce human-like atopic dermatitis in mice and mast cell’s DCIR may play a significant role in the development of AD. Therefore, DCIR may be a novel therapeutic target for AD.