Lack of Genetic Association between CDHR3 and Human Rhinovirus C Infection in Hong Kong Children Hospitalized for Respiratory Tract Infections
Sunday, March 4, 2018
South Hall A2 (Convention Center)
Ting Fan Leung, MD FRCPCH FAAAAI, Man Fung Tang, Yu Ping Song, Agnes Sze Yin Leung, Kin Pong Tao, Joseph Gar Shun Tsun, Gary Wing Kin Wong, Renee Wan Yi Chan
RATIONALE: CDHR3 encoding a newly identified receptor for human rhinovirus C (HRV-C) was reported to be associated with recurrent severe asthma exacerbations in young Danish children. However, HRV-C status was not tested in these subjects. This study investigated any association between CDHR3 and HRV-C infection in Hong Kong Chinese children.

METHODS: Children younger than 18 years old who were hospitalized for respiratory tract infections between April and September of 2015 were identified. RNA was extracted from their archived nasopharyngeal aspirate (NPA) samples for HRV detection and typing. cDNA was subjected to genotyping of 10 tagging single-nucleotide polymorphisms (SNPs) located within 5-kb both up-stream and down-stream from the top SNP rs6967330 of CDHR3 identified from a GWAS by TaqMan assays. Univariate and logistic regression analyses were performed to detect any relationship between HRV-C infection and SNPs of CDHR3.

RESULTS: 1064 Chinese patients, with mean age (SD) of 3.7 (3.5) years, were recruited. About three-fifths were males. HRV species were genotyped in 573 patients, and 320 (55.8%) had HRV-A, 47 (8.2%) had HRV-B and 206 (36.0%) had HRV-C. In total, 480 patients had complete data for both HRV genogroups and CDHR3 SNPs. Compared with HRV-A and HRV-B infections, there was no significant association between HRV-C infection and CDHR3 SNPs including the top SNP rs6967330 (P=0.795). Subgroup analysis for those aged under 6 years also yielded negative results.

CONCLUSIONS: Among Chinese children hospitalized with respiratory tract infections, this study cannot detect any significant association between HRV-C and CDHR3. (Funding: Direct Grant for Research [4054292], CUHK)