256:
Effect of prenatal supplementation of mothers with Lactobacillus johnsonii on offspring microbiome and RSV immunity
Saturday, March 3, 2018: 5:45 PM
S210C (Convention Center)
Wendy Fonseca, , , , ,
RATIONALE: The development of the immune system before and after birth may be altered by several extrinsic and intrinsic factors that could lead to severe disease predisposition in children who do not exhibit any known risk factor. Recently, the role of the microbiome and resulting metabolites have been an area of intense study in the development of lung disease, including viral infection and asthma. Respiratory syncytial virus (RSV) is a common pathogen that infects virtually all children by two years old, and is the leading cause of hospitalization of infants worldwide. While most children experience mild symptoms, some children progress to severe lower respiratory tract infection. These children with severe disease have a much higher risk to develop childhood wheezing later in life. Our group has shown previously, that mice supplemented with L. johnsoniibefore RSV-challenge showed decreased RSV immunopathology.

METHODS: In this work, we studied the effect of L. johnosonii prenatal supplementation of mothers and the effect in their offspring after RSV infection in 14 days old mice.

RESULTS: We observed that offspring from L-johnsonii supplemented mothers had reduced mucus deposition in airway and decreased expression of mucus relating genes (Gob5 and Muc5b). In lung draining lymph nodes we detected decreased production of Th2 cytokines. Finally, we observed different microbial communities in the gut of mice that were born from L. johnsonii supplemented mice that correlated to decreased disease pathology.

CONCLUSIONS: Together these data suggested that prenatal supplementation of mothers mediate airway mucosal protection against RSV infection in neonatal mice.